As a result of its quantification capability, real-time PCR has numerous applications in research facilities of:

Academia, where many academic studies use real-time PCR for numerous applications.

Pharmaceutical industry, where real-time PCR is  used in gene expression analysis and diagnostics in various drug development stages.

Molecular diagnostics to quantitatively detect and monitor cancer cells as well as viral and bacterial loads.

Agri-tech to address the regulatory requirement for monitoring genetically modified crops.

Forensics to enable accurate allocation of rare specimens to further investigation tools.

Bio-military and homeland security for quick and accurate detection of biological substances and their amount.

Food safety to aid in pathogen testing to maintain regulatory demands and avoid financial loss.